Genetic transfer and recombination in bacteria ppt
Many bacteria can acquire new genes by taking up DNA molecules e. The ability to deliberately transform the bacterium E. The first demonstration of bacterial transformation was done with Streptococcus pneumoniae and led to the discovery that DNA is the substance of the genes. The path leading to this epoch-making discovery began in with the work of an English bacteriologist, Fred Griffith. The cells of S. When grown on the surface of a solid culture medium, the capsule causes the colonies to have a glistening, smooth appearance.
These cells are called "S" cells. However, after prolonged cultivation on artificial medium, some cells lose the ability to form the capsule, and the surface of their colonies is wrinkled and rough "R". With the loss of their capsule, the bacteria also lose their virulence. Injection of a single S pneumococcus into a mouse will kill the mouse in 24 hours or so. But an injection of over million x 10 6 R cells is entirely harmless.
The capsule prevents the pneumococci from being engulfed and destroyed by scavenging cells — neutrophils and macrophages — in the body [ View ]. The R forms are completely at the mercy of phagocytes.
Pneumococci also occur in over 90 different types: The types differ in the chemistry of their polysaccharide capsule. Mice injected with a few S cells of, say, Type II pneumococci, will soon have their bodies teeming with descendant cells of the same type. The S-II cells remained true to their new type.
The process was named transformation. In pursuing Griffith's discovery, they found that they could bring about the same kind of transformation in vitro using an extract of the bacterial cells. Treating this extract with. But treating the extracts with DNase to destroy the DNA in them did abolish their transforming activity. So DNA was the only material in the dead cells capable of transforming cells from one type to another.
DNA was the substance of genes. Although the chemical composition of the capsule is determined by genes, the relationship is indirect. The phenotype of the pneumococci — the chemical composition of the polysaccharide capsule — is determined by the particular enzymes proteins used in polysaccharide synthesis. Unfortunately, the importance of their discovery was not sufficiently appreciated by scientists in general and the Nobel Committee in particular, and Avery died before their work could be honored with a Nobel Prize.
Nobel prizes are never given posthumously. As the donor replicates its chromosome, the copy is injected into the recipient. At any time that the donor and recipient become separated, the transfer of genes stops. Those genes that successfully made the trip replace their equivalents in the recipient's chromosome. The understanding of complex systems almost always has to await unraveling the details of some simpler system.
You may feel that trying to find out how one type of pneumococcus could be converted into another was an exceedingly specialized and esoteric pursuit. But Avery and his coworkers realized the broader significance of what they were observing and, in due course, the rest of the scientific world did as well.
By electing to work with a well-defined system: Attempting to understand the workings of complex systems by first understanding the workings of their parts is called reductionism. Some scientists and many nonscientists question the value of reductionism. They favor a holistic approach emphasizing the workings of the complete system. But the record speaks for itself.
From skyscrapers to moon walks, to computer chips to the advances of modern medicine, progress comes from first understanding the properties of the parts that make up the whole.
The late George Wald, who won the Nobel Prize in Physiology or Medicine for his discoveries of the molecular basis of detecting light [ Link ], once worried that his work was overly specialized — studying not vision, not the eye, not the whole retina, not even their rods and cones, but just the chemical reactions of their rhodopsins.
But he came to realize "it is as though this were a very narrow window through which at a distance one can see only a crack of light. As one comes closer, the view grows wider and wider, until finally through this same window one is looking at the universe.
I think this is the way it always goes in science, because science is all one. It hardly matters where one enters, provided one can come closer Transformation Conjugation Transduction Significance of genetic recombination in bacteria. Link to a discussion of cloning genes by transforming E. Streptococcus pneumoniae pneumococci growing as colonies on the surface of a culture medium.
The presence of a capsule around the bacterial cells gives the colonies a glistening, smooth S appearance. Pneumococci lacking capsules have produced these rough R colonies. Courtesy of Robert Austrian, J. Encapsulated left and nonencapsulated right pneumococci. The encapsulated forms produce smooth colonies above.
Discussion of restriction enzymes and examples of their use in making recombinant DNA screening for genetic diseases.
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